FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13442
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG138010.266329415265584No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA8546.3532212468382685No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG7145.311709567028716No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG5213.8759113227198334No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC2922.172295789317066No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT2491.8524029162327034No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC2481.8449635470912065No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG2231.6589793185537864No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG2101.562267519714328No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1951.4506769825918762No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1611.197738431780985No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT1481.1010266329415266No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG1451.0787085255170363No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT1060.7885731289986609No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG1030.7662550215741706No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG1020.7588156524326737No Hit
TACCCTTACGATGTTCCTGATTACGTTGACAAAGCTGAACCCTTCTGTCT920.6844219610177057No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC910.6769825918762089No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT880.6546644844517185No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA860.6397857461687249No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG820.6100282696027377No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT800.595149531319744No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT760.5653920547537569No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA720.5356345781877696No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG710.5281952090462729No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG650.4835589941972921No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA630.46868025591429846No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT630.46868025591429846No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG570.4240440410653176No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT560.41660467192382084No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT560.41660467192382084No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG550.4091653027823241No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA500.37196845707484005No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG490.36452908793334327No Hit
TACCCTTACGATGTTCCTGACAAAGCTGAACCCTTCTGTCTCTTATACAC480.3570897187918465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT470.34965034965034963No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTCGGCAGCGTCAGATGTTGAC460.34221098050885285No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG420.31245350394286564No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG400.297574765659872No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT370.27525665823538165No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG360.2678172890938848No Hit
TACCCTTACGATGTTCCTGACAACGCTGAACCCTTCTGTCTCTTATACAC340.2529385508108912No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACAAAGCTGAACCCTTC310.2306204433864008No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT290.21574170510340723No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT280.20830233596191042No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT280.20830233596191042No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA270.20086296682041363No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG270.20086296682041363No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG270.20086296682041363No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG260.19342359767891684No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA250.18598422853742003No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG250.18598422853742003No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT240.17854485939592324No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG230.17110549025442642No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC230.17110549025442642No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC220.16366612111292964No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG220.16366612111292964No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT220.16366612111292964No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG220.16366612111292964No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGT210.15622675197143282No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA210.15622675197143282No Hit
TACCCTTACGATGTTCCTGATTAGTTTCCACATCTTAACCCTTCTGTCTC210.15622675197143282No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG210.15622675197143282No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT200.148787382829936No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT200.148787382829936No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT190.14134801368843922No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG190.14134801368843922No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG190.14134801368843922No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG180.1339086445469424No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG170.1264692754054456No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG170.1264692754054456No Hit
TACCCTTACGATGTTCCTGATGACAAAGCTGAACCCTTCTGTCTCTTATA170.1264692754054456No Hit
TACCCTTACGCTGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT170.1264692754054456No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT160.11902990626394881No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT160.11902990626394881No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT160.11902990626394881No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGCTCGAAGATTTCGTTGGGGA150.11159053712245202No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAGGCCTGTCAGGCCAAGCTTG150.11159053712245202No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT150.11159053712245202No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG140.10415116798095521No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)