FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG618848.11071373036852No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA4113.1954594930803917No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG3312.5734722438190016No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG2932.2780283004198414No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG2501.9437101539418442No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1441.1195770486705023No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1140.886331830197481No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG920.7152853366505987No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG750.5831130461825532No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG720.5597885243352512No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT650.5053646400248796No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT650.5053646400248796No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG600.4664904369460426No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG540.4198413932514383No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA510.3965168714041362No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC480.3731923495568341No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG470.3654175089410667No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT420.32654330586222985No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG400.3109936246306951No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT400.3109936246306951No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT370.2876691027833929No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG360.2798942621676256No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG360.2798942621676256No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA340.2643445809360908No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG320.24879489970455604No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG310.24102005908878868No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG300.2332452184730213No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG300.2332452184730213No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG300.2332452184730213No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA290.2254703778572539No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG280.21769553724148655No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG280.21769553724148655No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG280.21769553724148655No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT280.21769553724148655No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT270.20992069662571916No Hit
CTGTAAAGATAAATGGGGCATCTAAAGAGTATGGAGCTGGGATTGACTCG270.20992069662571916No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG260.20214585600995177No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG250.19437101539418442No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG250.19437101539418442No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT240.18659617477841706No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC220.17104649354688228No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG220.17104649354688228No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA220.17104649354688228No Hit
CTGTAAAGATCAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG220.17104649354688228No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT200.15549681231534754No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT200.15549681231534754No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG190.14772197169958015No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG180.1399471310838128No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG180.1399471310838128No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG170.1321722904680454No Hit
ATGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG170.1321722904680454No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG160.12439744985227802No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG160.12439744985227802No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG160.12439744985227802No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA160.12439744985227802No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT160.12439744985227802No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG150.11662260923651065No Hit
CTGGAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG150.11662260923651065No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT140.10884776862074327No Hit
TACCCTTACGATGTTCCTGATTATGTTCCTGATTACGCTTGGGCTCGAAG140.10884776862074327No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG140.10884776862074327No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGAATCG140.10884776862074327No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT140.10884776862074327No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC140.10884776862074327No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT130.10107292800497589No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG130.10107292800497589No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG130.10107292800497589No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC130.10107292800497589No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT130.10107292800497589No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG130.10107292800497589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)