FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG8359.764939773125949No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA6797.940591743655713No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG6507.601450122792657No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG4925.753713016021518No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT2472.888551046661209No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG1812.116711495731493No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1151.3448719448017776No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT971.1343702490936733No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC961.122675710443223No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG850.9940357852882704No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG700.8186177055315168No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG640.7484504736288153No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG640.7484504736288153No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT620.7250613963279149No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT610.7133668576774647No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT600.7016723190270144No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC580.6782832417261139No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA550.6431996257747632No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG530.6198105484738627No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG510.5964214711729622No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG510.5964214711729622No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG510.5964214711729622No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT450.5262542392702607No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA450.5262542392702607No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG440.5145597006198105No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT420.4911706233189101No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT400.46778154601800953No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT400.46778154601800953No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT380.4443924687171091No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG350.4093088527657584No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG350.4093088527657584No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG330.38591977546485795No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT320.37422523681440767No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG310.36253069816395744No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG310.36253069816395744No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA300.3508361595135072No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG290.33914162086305694No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG280.3274470822126067No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG270.3157525435621565No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT260.3040580049117062No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT250.292363466261256No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT250.292363466261256No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA230.26897438896035547No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG220.25727985030990524No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA220.25727985030990524No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT210.24558531165945505No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG210.24558531165945505No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG210.24558531165945505No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG210.24558531165945505No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT200.23389077300900477No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC200.23389077300900477No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT190.22219623435855454No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG190.22219623435855454No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT180.2105016957081043No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG180.2105016957081043No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG170.19880715705765406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT160.18711261840720383No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT160.18711261840720383No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG160.18711261840720383No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG150.1754180797567536No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGCTCGAAGATTTCGTT140.16372354110630336No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA140.16372354110630336No Hit
TACCCTTACGATGTTCCTGATTACGCGTGTGCTCGAAGATTTCGTTGGGG140.16372354110630336No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT140.16372354110630336No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG130.1520290024558531No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT130.1520290024558531No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGGCTCGAAGATTTCGTTG130.1520290024558531No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG130.1520290024558531No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT130.1520290024558531No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTCGCGTGGGCTCGAAGATTTCGTTGGGGA120.14033446380540288No Hit
TACCCTTACGATGTTCCTGATTAGTGGGCTCGAAGATTTCGTTGGGGACT110.12863992515495262No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG110.12863992515495262No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT100.11694538650450238No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG100.11694538650450238No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGT100.11694538650450238No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTTGGG90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTTGGT90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT90.10525084785405216No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGTGGGCTCGAAGATTTCGTT90.10525084785405216No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG90.10525084785405216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)