FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47557
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3362670.70673087032404No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG9862.0733015118699667No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA7851.6506507979897806No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG7021.4761233887755747No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG4861.0219315768446284No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG3870.8137603297096117No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3210.6749794982862669No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2400.5046575688121623No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG2350.4941438694619089No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1850.38900687595937505No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1770.37218495699896964No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG1630.3427465988182602No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1600.33643837920810815No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT1590.3343356393380575No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG1490.3133082406375507No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT1410.2964863216771453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG1350.2838698824568413No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC1290.2712534432365372No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG1150.24181508505582774No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC1010.21237672687511827No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1010.21237672687511827No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT960.2018630275248649No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG820.17242466934415543No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG770.16191096999390203No Hit
TACCCTTACGATGTTCCTGATTACGTCAGTTGACAAAGCTGAACCCTTCT760.15980823012385137No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG750.1577054902538007No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG690.14508905103349665No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG680.14298631116344596No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG620.13036987194314192No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG620.13036987194314192No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG610.12826713207309123No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG610.12826713207309123No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA600.12616439220304057No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG600.12616439220304057No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG590.1240616523329899No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG580.12195891246293922No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT580.12195891246293922No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA570.11985617259288853No Hit
TACCCTTACGATGTTCCTGATTACGCTGTTACAAAGCTGAACCCTTCTGT550.11565069285278719No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG550.11565069285278719No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG540.11354795298273651No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG530.11144521311268582No Hit
CTGTAAAGATAAATGGGTCATCTACAGAGTATGGAGCTGGGATTGACTCG520.10934247324263516No Hit
TACCCTTACGATGTTCCTGACAAAGCTGAACCCTTCTGTCTCTTATACAC510.10723973337258448No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT490.10303425363248311No Hit
TACCCTTACGATGTTCCTGATTACGCGCACCACCACCACCATGTTGACAA490.10303425363248311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)