FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50080
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3472869.3450479233227No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG11452.2863418530351436No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG8961.7891373801916934No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA6581.3138977635782747No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA6251.248003194888179No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA5821.1621405750798721No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG3180.6349840255591054No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3140.6269968051118211No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG2740.5471246006389776No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA2720.5431309904153354No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC2660.5311501597444089No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2220.4432907348242811No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG2200.43929712460063897No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1980.3953674121405751No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1960.39137380191693294No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1870.37340255591054317No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1580.31549520766773165No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG1460.2915335463258786No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG1270.253594249201278No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC1230.24560702875399362No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT1200.23961661341853036No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1090.2176517571884984No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG1000.1996805111821086No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA920.18370607028753994No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG820.16373801916932906No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG810.161741214057508No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG800.1597444089456869No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG770.15375399361022363No Hit
CTGTAAAGATAAATGGGTCATCTGCCTGTTGACAAAGCTGAACCCTTCTG760.15175718849840256No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG700.13977635782747602No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT670.13378594249201278No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG660.1317891373801917No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG660.1317891373801917No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG620.12380191693290735No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG620.12380191693290735No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGACTCG600.11980830670926518No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT590.1178115015974441No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG590.1178115015974441No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG580.115814696485623No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA570.11381789137380191No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG560.11182108626198084No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG550.10982428115015974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)