FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9892
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG9749.846340477153255No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA5915.974524868580671No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG5555.6105944197331175No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG3703.740396279822079No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG2592.6182773958754546No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT2372.395875454913061No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1531.5467044076021028No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1391.405175899716943No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1381.3950667205822886No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1031.0412454508693894No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC1001.0109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG991.0008087343307723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT890.8997169429842297No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG840.8491710473109584No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT790.798625151637687No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA740.7480792559644156No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG720.7278608976951072No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT710.7177517185604528No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT640.646987464617873No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT630.6368782854832188No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG560.5661140315406389No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT560.5661140315406389No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT520.5256773150020219No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC520.5256773150020219No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT520.5256773150020219No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG480.4852405984634048No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA460.4650222401940962No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG450.45491306105944196No Hit
TACCCTTACGATGTTCCTGATTACGGTCAGTTGACAAAGCTGAACCCTTC410.4144763445208249No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG380.3841488071168621No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA380.3841488071168621No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG370.3740396279822078No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA350.35382126971289934No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG350.35382126971289934No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC330.3336029114435908No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG330.3336029114435908No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG330.3336029114435908No Hit
TACCCTTACGATGTTCCTGATTGACAAAGCTGAACCCTTCTGTCTCTTAT330.3336029114435908No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG320.3234937323089365No Hit
TACCCTTACGATGTTCCTGATGACAAAGCTGAACCCTTCTGTCTCTTATA310.31338455317428227No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT310.31338455317428227No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC310.31338455317428227No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG300.303275374039628No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT290.2931661949049737No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT290.2931661949049737No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT290.2931661949049737No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG270.2729478366356652No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG250.2527294783663567No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT250.2527294783663567No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT240.2426202992317024No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT240.2426202992317024No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG230.2325111200970481No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG230.2325111200970481No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA230.2325111200970481No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT210.2122927618277396No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG190.19207440355843106No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG190.19207440355843106No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG190.19207440355843106No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT180.1819652244237768No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAACGGAAGGCG180.1819652244237768No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT180.1819652244237768No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC170.17185604528912252No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG170.17185604528912252No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA170.17185604528912252No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT170.17185604528912252No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA160.16174686615446826No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT160.16174686615446826No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTTGTGTTAACGGACGGCT160.16174686615446826No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG160.16174686615446826No Hit
TACCCTTACGATGTTCCTGATTACTCTGGGCTCGAAGATTTCGTTGGGGA150.151637687019814No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG150.151637687019814No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT150.151637687019814No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG150.151637687019814No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG140.14152850788515972No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT140.14152850788515972No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT140.14152850788515972No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT130.13141932875050547No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGT130.13141932875050547No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA130.13141932875050547No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT120.1213101496158512No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA120.1213101496158512No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG120.1213101496158512No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG120.1213101496158512No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTTGTGTTAACGGAAGGCG120.1213101496158512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG110.11120097048119691No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA110.11120097048119691No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG110.11120097048119691No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT110.11120097048119691No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC110.11120097048119691No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG100.10109179134654267No Hit
TACCCTTACGCTGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG100.10109179134654267No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG100.10109179134654267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)