FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1856.821533923303836No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1686.1946902654867255No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1686.1946902654867255No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1354.977876106194691No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG682.5073746312684366No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG511.8805309734513276No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG401.4749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG391.4380530973451326No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT371.364306784660767No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT351.2905604719764012No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC301.1061946902654867No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG281.0324483775811208No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT270.995575221238938No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC250.9218289085545722No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG240.8849557522123894No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA190.7005899705014749No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG180.6637168141592921No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG170.6268436578171092No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT160.5899705014749262No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT160.5899705014749262No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT150.5530973451327433No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT130.47935103244837757No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG120.4424778761061947No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA120.4424778761061947No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT110.4056047197640118No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA110.4056047197640118No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG110.4056047197640118No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT100.3687315634218289No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG90.33185840707964603No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG80.2949852507374631No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.2949852507374631No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG80.2949852507374631No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT80.2949852507374631No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA70.2581120943952802No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG70.2581120943952802No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGCTCGAAGATTTCGTTG70.2581120943952802No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTTGGG70.2581120943952802No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT70.2581120943952802No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATCCGATTCGTGTTAAAT60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGCACCACCACCACCATGTTGACAA60.22123893805309736No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.18436578171091444No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT50.18436578171091444No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT50.18436578171091444No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT40.14749262536873156No Hit
TACCCTTACGCTGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGGCTCGAAGATTTCGTTGGG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTGCTCGAAGCTTTCGTTG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGATTCGTGGTAAAT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATACGAGTCGTGGTAAAT40.14749262536873156No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGAAAAT30.11061946902654868No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGATGGGATTGACTCG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGAGGGCTCGAAGATTTCGTTG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGACAGACGAGTCGTGGTAAAT30.11061946902654868No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCCGTGGGCTCGAAGATTTCG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGTCTCGAAGATTTCGTTG30.11061946902654868No Hit
TACCCTTACGCTGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATACGAGTCGTGTTAAAT30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTCGCGTGGGCTCGAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGGCGTGGTAAAT30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGT30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGCCGATTCGTGTTAAAT30.11061946902654868No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGGTCGAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCGCGAAGATTTCGTTGGGGA30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGAATTCGTTT30.11061946902654868No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG30.11061946902654868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)