FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP1B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP1B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG2877.16246568505116No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG2506.239081607187423No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA2245.59021712003993No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT2175.415522835038682No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1062.6453706014474667No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT1032.570501622161218No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1012.5205889693037187No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT972.42076366358872No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA801.996506114299975No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG751.8717244821562267No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG691.7219865235837284No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT661.6471175442974793No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG531.3226853007237334No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG390.9732967307212379No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC380.9483404042924881No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC370.9233840778637383No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG370.9233840778637383No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG360.8984277514349888No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG290.7237334664337409No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG280.6987771400049912No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG270.6738208135762416No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA260.6488644871474919No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG230.5739955078612428No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT220.5490391814324931No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT220.5490391814324931No Hit
TACCCTTACGATGTTCCTGATTACCGGCAGCGCAGTTGACAAAGCTGAAC210.5240828550037434No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG210.5240828550037434No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG200.49912652857499373No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGCTCGAAGATTTCGTTG180.4492138757174944No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT180.4492138757174944No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT150.3743448964312453No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG130.32443224357374595No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT130.32443224357374595No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG130.32443224357374595No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT130.32443224357374595No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG120.2994759171449962No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT120.2994759171449962No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG110.27451959071624654No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG100.24956326428749687No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT90.2246069378587472No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG90.2246069378587472No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.2246069378587472No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG90.2246069378587472No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGGCTCGAAGATTTCGTTG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCGTGTGCTCGAAGATTTCGTTGGGG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG80.19965061142999752No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG70.1746942850012478No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG70.1746942850012478No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGCTCGAAGATTTCGTT70.1746942850012478No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG70.1746942850012478No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT70.1746942850012478No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGG60.1497379585724981No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT60.1497379585724981No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA60.1497379585724981No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG60.1497379585724981No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTTGGG50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACTAGTTGACAAAGCTGAACCCTTCTGT50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGTCGTGGGCTCGAAGATTTCGTTGGG50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACCAGCGTCAATGTGACAAAGCTGAACC50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC50.12478163214374843No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA50.12478163214374843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)