FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_MalKPathoPool4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_MalKPathoPool4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25687
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1557960.64935570522054No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT6992.721220851014132No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT5822.265737532604041No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG4451.732393817884533No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT2821.0978315879627827No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT2801.090045548331841No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACGTTCGT2611.0160781718378948No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT2300.895394557558298No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1970.7669249036477596No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1960.7630318838322887No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACCTTCGT1900.7396737649394636No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1620.6306692101062794No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG1380.5372367345349788No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1370.5333437147195079No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACGTTCGT1320.5138786156421536No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG1250.48662747693385755No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACGTTCGT1110.4321251995172655No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT1030.4009810409934987No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTCACCTTCGT1000.38930198154708606No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACCTTCGT950.3698368824697318No Hit
CTGTAAAGATAAATGGGGCATCTAAAGAGTATGGAGCTGGGATTGACTCG930.36205084283879No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG890.3464787635769066No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG830.3231206446840814No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT820.3192276248686106No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACGTTCGT790.307548565422198No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACCTTCGT790.307548565422198No Hit
CTGTAAAGATCAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG780.30365554560672714No Hit
CGGTTCGTGCGCTTCTGTGATGCGTTCGAGATTCCGATCGTGACGTTCGT750.2919764861603146No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG740.28808346634484366No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT730.2841904465293728No Hit
ATGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG720.280297426713902No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG700.27251138708296024No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG620.24136722855919338No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTGACGTTCGT610.2374742087437225No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATTCCGATCGTGACCTTCGT600.23358118892825164No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTGACCTTCGT590.22968816911278078No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACGTTCGT520.20243703040448477No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG490.19075797095807218No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACCTTCGT460.1790789115116596No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGACTCG460.1790789115116596No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG410.1596138124343053No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT400.15572079261883442No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGAATCG370.14404173317242183No Hit
CTGTAAAGATAAATGGGTCATCTACAGAGTATGGAGCTGGGATTGACTCG340.13236267372600927No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACGTTCGT320.12457663409506754No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTCACCTTCGT320.12457663409506754No Hit
CTGTAAAGATAAAGGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG310.12068361427959669No Hit
CGGTTCGTCCGCTTCTGTGATGCGTTCGAGATTCCGATCGTGACGTTCGT310.12068361427959669No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG310.12068361427959669No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT310.12068361427959669No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTGTGGAGCTGGGATTGACTCG310.12068361427959669No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATTGAGCTGGGATTGACTCG290.11289757464865496No Hit
CTGTAAAGATAAAAGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG270.10511153501771324No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGATATCCCGATCGTGACGTTCGT270.10511153501771324No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTCACCTTCGT260.10121851520224238No Hit
CTGTAATGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG260.10121851520224238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)