FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_MalKPathoPool2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_MalKPathoPool2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55994
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3307359.06525699182055No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT25664.582633853627175No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT17533.1306925742043794No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT10521.8787727256491766No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT9681.728756652498482No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT9111.6269600314319392No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG7401.3215701682323107No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT6751.205486302103797No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACCTTCGT5200.9286709290281102No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACCTTCGT4040.7215058756295317No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT3870.6911454798728435No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT3300.5893488588063007No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATTCCGATCGTGACCTTCGT3150.5625602743151051No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTGACGTTCGT2820.5036253884344751No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACGTTCGT2710.4839804264742651No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACGTTCGT2620.46790727577954777No Hit
CGGTTCGTGCGCTTCTGTGATGCGTTCGAGATTCCGATCGTGACGTTCGT2610.4661213701468015No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG2190.3911133335714541No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACCTTCGT1940.34646569275279493No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG1700.30360395756688213No Hit
CGGTTCGTGCGCTTCTGCGACGCATTCGAGATCCCGATCGTGACGTTCGT1650.29467442940315036No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT1630.29110261813765764No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACCTTCGT1620.28931671250491126No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTCACCTTCGT1550.27681537307568665No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGATATCCCGATCGTGACGTTCGT1400.2500267885844912No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1320.23573954352252027No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG1200.21430867592956387No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGACATCCCGATCGTGACCTTCGT1140.2035932421330857No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1140.2035932421330857No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATTCCGATCGTGACGTTCGT1130.20180733650033933No Hit
CGGTTCGTCCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACGTTCGT1100.19644961960210025No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1080.1928778083366075No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1030.18394828017287568No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGACATTCCGATCGTGACCTTCGT980.17501875200914382No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACGTTCGT980.17501875200914382No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG970.17323284637639746No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG970.17323284637639746No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG950.16966103511090472No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAAATTCCGATCGTGACGTTCGT940.16787512947815836No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACGTTCGT920.16430331821266564No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTGACCTTCGT890.15894560131442653No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTCACCTTCGT800.14287245061970927No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG800.14287245061970927No Hit
CGGTTCGTCCGCTTCTGTGATGCGTTCGAGATTCCGATCGTGACGTTCGT780.13930063935421652No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATCCCGATCGTGACATTCGT760.13572882808872377No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTCACCTTCGT710.12679929992499198No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACTTTCGT700.1250133942922456No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATTCCGATCGTCACCTTCGT690.12322748865949923No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG690.12322748865949923No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG680.12144158302675287No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG660.11786977176126014No Hit
CGGTTCGTACGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT650.11608386612851378No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGACTCG640.11429796049576739No Hit
CTGTAAAGATAAATGGGTCATCTACAGAGTATGGAGCTGGGATTGACTCG620.11072614923027467No Hit
CGGTTCGTCCGCTTCTGCGACGCATTCGAGATCCCGATCGTGACGTTCGT610.10894024359752831No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT610.10894024359752831No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG610.10894024359752831No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG600.10715433796478194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)