FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_LowSpecHamPathoPool2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_LowSpecHamPathoPool2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT6127.727272727272727No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG4520.454545454545457No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG94.090909090909091No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG31.3636363636363635No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT20.9090909090909091No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.9090909090909091No Hit
ACACCCAAGCAGATCTACAACCGCATGCAGCACGTCTATGCCGACCAGCA20.9090909090909091No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA20.9090909090909091No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.9090909090909091No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT20.9090909090909091No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGACATTT20.9090909090909091No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.9090909090909091No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.9090909090909091No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.9090909090909091No Hit
GATGCGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGAGTCAGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCAAAGTTTTTTGTG10.45454545454545453No Hit
ACGCCCAAGCAGATTTACGTCCGCTGGCAATCCCTGGTTCAGCCGCAAGT10.45454545454545453No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GAGGAGTCCGTGTCTAACATGGAAACTTGATTTCAGCAGACGTGGCACTT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGTCATTT10.45454545454545453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGTTGGGATTGACTCG10.45454545454545453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGCCTCG10.45454545454545453No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGCGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA10.45454545454545453No Hit
GAAGCGTCCGCGTCTAACATGAACCCTTTATGGCCTGATACGTGTCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCCCCTGGCTCTCGAATTTCTTTGTT10.45454545454545453No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATGTCCTAATACGTATCATTT10.45454545454545453No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT10.45454545454545453No Hit
GATAAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGAATCTT10.45454545454545453No Hit
GAGCAATAAGACGCTCCCGTGTACGATTTTTTAGTTCGTACGTGGCATTG10.45454545454545453No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG10.45454545454545453No Hit
ACACCCAAGCAGATTTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT10.45454545454545453No Hit
GATGCGTCAGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT10.45454545454545453No Hit
ACGCCCAAGCAGATCTACAATCGCATGCAGATCGTCTATGTCGTTCAGCA10.45454545454545453No Hit
TACCCTTACGCTGTTCCTGCTTACGCCGTGGGCTCTAGCATTTCTTTGGT10.45454545454545453No Hit
ACGCCCAAGCAGATTTACAACCGCATGCAGAAAGTCTATGCAGACCAGCA10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTTGAAGATTTCGTTGGGGA10.45454545454545453No Hit
ACACCCAAGCAGATCTACGAGTCGTGGTAATTTTAATCTGGCTGTGGTCC10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATACAACATTTATTGCATGATACGTAGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGCAGATTTCGTTGGGGA10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT10.45454545454545453No Hit
ACGCCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGACCAGCA10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
ACACCCAAGCAGATCTACGCGAGCTCCCTTGCCTCAATCCGAGTTCCTAG10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGCACAGCCTGCCTAA10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGATCGCACATTGCGTTGGTGG10.45454545454545453No Hit
ACACCCAAGCAGATCTACCCACCGTAATACGTCTCAGTCCAGCCTTAAGT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATCGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCGCAGATTTTTTGGTGGGC10.45454545454545453No Hit
GATGCGTCCGCGTCTAACATGAACCCTCTATTTCATGATACGTGTCATTT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGAGACAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTTCA10.45454545454545453No Hit
ACACCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGTCCAGCA10.45454545454545453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG10.45454545454545453No Hit
CTGTAAAGATAAATGGGTCATCTAACCGTTCTGCCTCTGGTCGCCACTTT10.45454545454545453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG10.45454545454545453No Hit
CTGCAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.45454545454545453No Hit
GATTGAGTCAGCGTCGCGGCGTACTTTATTTACGGCCAGAATCTCGACCT10.45454545454545453No Hit
GATGCGTCCGCGTCTAACATGCACCCTCGATTTCATGATACGTGTCATTG10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTGTCTCTACTATTTCGTTTGGGAC10.45454545454545453No Hit
GAGGAGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT10.45454545454545453No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGCGTCAGCGTCTCGGCGTACTTTATTTACGGCCAGAAACTCTACCTG10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGGGGGGCCGAAAGATTCCTTTGGG10.45454545454545453No Hit
GATGAGTCAGCGTCGAACACGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG10.45454545454545453No Hit
ACACCCAAGCAGATCTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC10.45454545454545453No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGCTTACGCTTTGGCTCGAAGATTTCGTTGGGT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCACTCAA10.45454545454545453No Hit
GATGAGGCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCGCGAAGATTTCGGTGGGG10.45454545454545453No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGCGAGTGACTCG10.45454545454545453No Hit
GAGGCGTCCGCGTCTAACATGTACCCTTTATGCCCTGATTCGGGTCAGTT10.45454545454545453No Hit
GATGAGTCAGCGTCCAACATGCACCATCTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGCGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT10.45454545454545453No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTGGGGACTCTGT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACACGCAACATTTAATGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT10.45454545454545453No Hit
GATGAGTCATCGTCGAACATGCAACATTTTTTGCATGATACGTTGCATTT10.45454545454545453No Hit
GATGAGTCAGCGTAGAACATGCAACATTTAATGCATGATACGTGGCATTT10.45454545454545453No Hit
CTGTAAAGATAAATGGGCCCACTAAAGAGTATGGAGCTGGGATTGACTCG10.45454545454545453No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCATGATACGTGGCATTT10.45454545454545453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)