FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_JS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_JS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA653.237051792828685No Hit
ACACCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA623.087649402390438No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT613.037848605577689No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG582.888446215139442No Hit
ACGCCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGACCAGCA582.888446215139442No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAATCTGTTCGTGGCCAGCTT552.739043824701195No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAATCTGTTTGTGGCCAGCTT482.3904382470119523No Hit
ACACCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGACCAGCA401.9920318725099602No Hit
ACGCCCAAGCAGATTTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC381.8924302788844622No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT371.8426294820717133No Hit
ACGCCCAAGCAGATCTACAACGATCCGGCCAATCTGTTTGTGGCTAGCTT361.7928286852589643No Hit
ACACCCAAGCAGATTTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC351.743027888446215No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT351.743027888446215No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAATCTGTTCGTGGCCAGCTT341.693227091633466No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT331.643426294820717No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT271.344621513944223No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGCTT271.344621513944223No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGCTT271.344621513944223No Hit
ACACCCAAGCAGATCTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC261.294820717131474No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAATCTGTTCGTGGCCAGCTT231.1454183266932272No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT211.045816733067729No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAATCTGTTTGTGGCCAGCTT200.9960159362549801No Hit
ACGCCCAAGCAGATCTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC200.9960159362549801No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAATCTGTTCGTGGCCAGCTT190.9462151394422311No Hit
ACACCCAAGCAGATCTACAACGATCCGGCCAATCTGTTTGTGGCTAGCTT190.9462151394422311No Hit
ACACCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT180.8964143426294822No Hit
ACGCCCAAGCAGATTTACGCGAGATACACTGCCAGAAATCCGCGTGACTC140.697211155378486No Hit
ACACCCAAGCAGATTTACGCGAGATACACTGCCAGAAATCCGCGTGACTC140.697211155378486No Hit
ACGCCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGCTT130.647410358565737No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT120.5976095617529881No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAATCTGTTTGTGGCCAGCTT120.5976095617529881No Hit
ACGCCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT100.49800796812749004No Hit
ACGCCCAAGCAGATTTACAACGATCCGGCCAATCTGTTTGTGGCTAGCTT100.49800796812749004No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAATCTGTTTGTGGCCAGCTT90.4482071713147411No Hit
ACGCCCAAACAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA70.348605577689243No Hit
ACACCCAAGCAGATTTACAACCGCATGCAGAACGTGTACGCCGACCAGCA60.29880478087649404No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTCGCCAGCTT60.29880478087649404No Hit
ACACCCAAGCAGATCTACGCGAGATACACTGCCAGAAATCCGCGTGACTC50.24900398406374502No Hit
ACACCCAAGCAGATTTACAACCGCCTGCAGAACGTCTATGCCGACCAGCA50.24900398406374502No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAATCTGTTCGTCGCCAGTTT50.24900398406374502No Hit
ACACCCAAGCAGATTTACAACGATCCGGCCAATCTGTTTGTGGCTAGCTT50.24900398406374502No Hit
ACACCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGCTT40.199203187250996No Hit
ACACCCAAGCAGATTTACGGCAACCTGAGTCGTTCGGTCCAACCGCAAGT40.199203187250996No Hit
ACACCCAAGCAGATTTACGGCAACCTGAGTCGTTCGGTCCAGCCGCAAGT40.199203187250996No Hit
ACGCCCAAGCAGATCTACGCGAGATACACTGCCGAAATCCGCGTGACTCA40.199203187250996No Hit
ACACCCAAACAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT40.199203187250996No Hit
ACGCCCAAGCAGATCTACAACCGCCTGCAGAACGTCTATGCCGACCAGCA40.199203187250996No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAATCTGTTTGTGGCTAGCTT40.199203187250996No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCTACCAGCA40.199203187250996No Hit
ACACCCAAGCAGATCTACAACAGCATGCAGAACGTCTATGCCGACCAGCA40.199203187250996No Hit
ACGCCCAAGCAGATCTACAACAGCATGCAGAACGTCTATGCCGACCAGCA40.199203187250996No Hit
ACACCCAAGCAGATCTACGGCAACCTGAGTCGTTCGGTCCAGCCGCAAGT40.199203187250996No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTCGCCAGCTT40.199203187250996No Hit
ACGCCCAAGCAGATCTACGCGAGATACACTGCCAGAAATCCGCGTGACTC30.14940239043824702No Hit
ACACCCAAACAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA30.14940239043824702No Hit
ACGCCCAAACAGATTTACGAGTCGTGGTAAATTTAATCTGGCTGTGGTCC30.14940239043824702No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG30.14940239043824702No Hit
ACACCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCTACCAGCA30.14940239043824702No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTGTACGCCGACCAGCA30.14940239043824702No Hit
ACACCAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTTCA30.14940239043824702No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACAAGCA30.14940239043824702No Hit
ACACCCAAGCAGATCTACAACGATCCGGCCAATCTGTTTGTGGCCAGCTT30.14940239043824702No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAATCTGTTTGTGGCCAGTTT30.14940239043824702No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTGGCCAGCTT30.14940239043824702No Hit
ACACCCAAGCAGATTTACAACCGCATGCATAACGTCTATGCCGACCAGCA30.14940239043824702No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTCGCCAGCTT30.14940239043824702No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTCGCCAGCTT30.14940239043824702No Hit
ACGCCCAAGCAGATCTACAACCGCATGCATAACGTCTATGCCGACCAGCA30.14940239043824702No Hit
ACGCCCAAGCAGATCTACTCGTCCCAGTCCAACCGCAAGTGACTGTCTCT30.14940239043824702No Hit
ACACCCAAGCAGATCTACAACCGCATGCAGCACGTCTATGCCGACCAGCA30.14940239043824702No Hit
ACACCCAAGCAGATCTACAACCGCATGCATAACGTCTATGCCGACCAGCA30.14940239043824702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)