FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_JS14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_JS14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3815.767634854771783No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT3715.352697095435685No Hit
ACGCCCAAGCAGATCTCCGAGCCCACGAGACCGAAGTATATCTCGTATGC177.053941908713693No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA83.319502074688797No Hit
ACGCCCAAGCAGATCTACGTTCAGTTGAGCCGCAAGTGACTGTCTCTTAT41.6597510373443984No Hit
ACGCCCAAGCAGATCGGAGAGCGTCGTGTAGGGTTGAGCCGCAAGTGACT41.6597510373443984No Hit
ACGCCCAAGCAGATCTACTAGTCGTGGTAAATTTAATCTGGCTGTGGTCC31.2448132780082988No Hit
ACGCCCAAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGAGCC31.2448132780082988Illumina Single End PCR Primer 1 (96% over 33bp)
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG31.2448132780082988No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.8298755186721992No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.8298755186721992No Hit
ACGCCCAAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGCAAG20.8298755186721992Illumina Single End PCR Primer 1 (96% over 33bp)
ACGCCCAAGCAGATCTACGAGTCGTGGTAAGTTGAGCCGCAAGTGACTGT20.8298755186721992No Hit
GATGAGTCCGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.8298755186721992No Hit
ACGCCCAAGCAGATCGGAAGACGTCGTGTAGGGAAAAGTGTGTTGAGCCG20.8298755186721992No Hit
ACGCCCACGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA20.8298755186721992No Hit
GATGAGTCAGCGTCGAAGCAGGCTGGAATCGATCGAGAACTGGCTGGACA20.8298755186721992No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGGCTCTT10.4149377593360996No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGTATGATACGTGGCATTT10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGCCCACGAGACAGTTGAGCCGCAAGTGACT10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAATGATTATGGCGCTGCTAATCACTTC10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCTTAGATTTCGTTGGGGA10.4149377593360996No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCTCCTCGTATTCTTTGCGGT10.4149377593360996No Hit
ACGCCCAAGCAGATCTACTGGTTGAGCCGCAAGTGACTGTCTCTTATACA10.4149377593360996No Hit
ACACCCAAGCAGCTCTAAAGAGCGTCGTTTGATTCGCCGCACGTTCCTGT10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGAGTGACGCG10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAATGATTTTGGAGTTGGGGTTGACCCG10.4149377593360996No Hit
ACGCCCAAGCAGATCTCCGAGCCCCCTCGACCCAAGTATATCTCCTATGC10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCTCCTAA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGCCGAGGTAAGATGAGCCGCAAGAGACTGT10.4149377593360996No Hit
ACGCCCAAGCAGATCTGAAGACGTCGTGTAGGGACAAGTGTGTTGAGCCG10.4149377593360996No Hit
ACGCCCAAACAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGCTCGAACGATTCTTTGGTTG10.4149377593360996No Hit
TACCCTTACTATGTTCCTGATTACGCTTTTGCTTCGCGGTTTTGGTGGGG10.4149377593360996No Hit
ACGCCCAAGCAGATCGGAAGAGCGTCGTGTGTTGAGCCGCAAGTGACTGT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTGATAGAAACTTTCGTTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTTCTCTAACATTTCGCTGGA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGTGCCTAGAGAGTAGCGGTT10.4149377593360996No Hit
ACGCCCAAGCATATCGGAGAGCGTCGTGTAGGGTTGAGCCGCAAGTGACT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGTCAATGTGGCGAAGCTTTAGGTG10.4149377593360996No Hit
GAAGAGGGCATCGTTCCAGGCGGCGGTACTGCACTTCTGCGCTCTTCCAC10.4149377593360996No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGGTGATCGCAGATTTAGTTGGGGA10.4149377593360996No Hit
ACGCCCAAGCAGATCTTGAGCCGCACGTGACTGTCTCTTATCCACATCTC10.4149377593360996No Hit
ACGCCCAAGCAGATCTAACGCTGCCGACGAAGTTGAGCCGCAAGTGACTG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTGATGGAAATTTTCGTTT10.4149377593360996No Hit
ACGCCCAAGCAGATCTAGCCGCAAGTGACTGTCTCTTATACACATCTCCG10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGCGAGATACACTGCCGAAATCCGCGTGACTCA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACCGCATTCAGAACGTCTATTCCGACCAGCA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGCTTACGCTGTGCTCTCAGATTTCGTTGGGGA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCACGAAGAAACCGTCGG10.4149377593360996No Hit
ACGCCCAAGCAAGTGACTGTCTCTTCTACAAATCTCCGAGCCCACGAGAC10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGCCCACGAGACGAGCCGCAAGTGACTGTCT10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGTCGTGGTAACTTTAATCTGGCTGTGGTCC10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCGGAGTTCGCGGGGGG10.4149377593360996No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGAGATTCCTATGTTGCCTCTG10.4149377593360996No Hit
GATGAGTCCGCGTCTAACATGCACCCTCTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTAAGCCTAGCCCGACGAGTTCTGGTAGGA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACCGCATGCATAACGTGTACGCCGACCAGCA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACAGCCTTCAGCACGTCTATTCCGACCAGAA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGGGGGTCGAAGATTTCTTGGTGTTAG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCCGAAAGATTCGTTGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCGCGGGCCCCAAAAGTTCTTTGGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATACGATTCGTGTTAAAT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGCGGATGTCTCTGTG10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGGATGGAAAAGGGATAGACTCG10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACCGCCTTCAGCACGTCTATTCCGACCAGCA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT10.4149377593360996No Hit
ACGCCCAAGCAGATCTTAGAGCGTCGTGTAGGTTTGAGCCGCAATTGACT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGTGGTTTCGACTATTTCGTTTGGGAC10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACAGCCTGCAGAACGTCTATGCCGACCAGCA10.4149377593360996No Hit
GAGGAAGGAATCGTGCGCTTTTGCTTCGCCAAGCAGGACGACACCTTGCG10.4149377593360996No Hit
ACGCCCAAGCAGATCACGTGTGGAAAGGTTGAGCCGCAAGTGACTGTCTC10.4149377593360996No Hit
TACACTTACGATGTTCCTGATTAAGCTCGACGATTTCGTTTGTTACTCTC10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATCGAGCTGGGATTGACTCG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCGA10.4149377593360996No Hit
ACGCCCAAGCAGATCTGAGAGCGTCGGGTAGGGAAAGAGTGTTTGAGCCG10.4149377593360996Illumina Single End PCR Primer 1 (96% over 25bp)
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGAGTTCGGTGGGGA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACCGCATTCAGAACGTCTATGCCGACCAGCA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCATCTCGTGGCTCTAAGATTTCGTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCTCGGAGTTCGTGGGGGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA10.4149377593360996No Hit
TACCCTAACGATGTTCCTGATATCGTCTAGCCAGCCGATTCGTGGTAAAT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACCTGGCCTCGACTATTTCGTTTGGGAC10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG10.4149377593360996No Hit
ACGCCCAAGCAGATCTCCGATCCCACGAGACCGAAGTATATCTCGTATGC10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGAGCTCTAATATTTCTTTGGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCGAGGTCAGTAACAGCCCGCCGCC10.4149377593360996No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGCCTCG10.4149377593360996No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.4149377593360996No Hit
ACGCCAAGCAGATCTACGAGCCCACGAGACAGTTGAGCCGCAAGTGACTG10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGCCGTGGTAAATTTAATCTGGCTGTGGTCC10.4149377593360996No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGCTCGCACATTTCGTTGGGT10.4149377593360996No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGCAGGTTTCGTTGGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGGTTCTCGCAGATTTAGTTGGGGG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGGATAATGCCTCGCCAATTTCCCTAA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATACCGTCTAGCCATCCGATTCGTGTTAAAG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGTATCTCGAAGGTTTCGCTG10.4149377593360996No Hit
GATGAGTCAGCGTCGAACATGCAACCTCTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATGACAAATCTGAACCTTTCTGTCTCGTATA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGATGGGGTCGCTG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAATATTACGTTGT10.4149377593360996No Hit
ACGCCCAAGCAGATCTACCGCAAGTGACTGTCTCTTATACACATCTCCGA10.4149377593360996No Hit
GACGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTATGTCTCGAAGCTTTCGCTG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTGCTCTCAGCATTCTTCTTTGACTCTGTC10.4149377593360996No Hit
ACGCCCAAGCAGATCATAGAGAGTCGTGTAGGGTTTAGCCACAAGGGAAT10.4149377593360996No Hit
ACGCCCAAGCAGATCTCCGCGCCCACTTGAGCCGCAAGTGACTGTCTCTT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGAGATTTCCTGCGGGCCTCT10.4149377593360996No Hit
ACACCCAAGCAGATCTAAAGAGCGTCTTTGAGTTCAAGATTGTGTGAGTC10.4149377593360996No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTGTACGCCGACCAGCA10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTAACACAGGGGCTCTCTTATTTCTTTTGT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCGTTGTCTCTACGATTTCGT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCTAACGTTGCGTTGGCTA10.4149377593360996No Hit
ACGCCCAAGCAGATCTACCGCAGTCAGTCCAGCCGCAAGTGACTGTCTCT10.4149377593360996No Hit
ACGCCCAAGCAGATCTACGAGTCGTGTCAAGTTTAGCCGCAAGTGACTGT10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAGGATTTCGTTG10.4149377593360996No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGAGTTCTCGAAGCTTTCGTTG10.4149377593360996No Hit
ACGCCCAAGCAGATCTCCGAGCCCACGAGACCGAAGTATATCTCGTATGG10.4149377593360996No Hit

[FAIL]Adapter Content

Adapter graph