FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_JS11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_JS11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT6935.38461538461539No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3015.384615384615385No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT31.5384615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT21.0256410256410255No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT21.0256410256410255No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT21.0256410256410255No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG21.0256410256410255No Hit
TGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCGGGGGGTTCTAATATTGCTTTGGG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGGGCCGCGATTTAGAAGCTTGCGTCT10.5128205128205128No Hit
GATGCGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGGAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTCGCCAGTTT10.5128205128205128No Hit
GATGCGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.5128205128205128No Hit
TACCCTTACTATGTTCCTGATCTAGCTCTTCGAGATCGGTTGTTGCCCCT10.5128205128205128No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGCGGCGTACTTTATTTACGGCCAGAAACTCGACCTG10.5128205128205128No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATTCCGATCGTGACCTTCGT10.5128205128205128No Hit
GATGAGTCATCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATAAGTGGCATTT10.5128205128205128No Hit
AATGCGTCCGCGTATAACATGAACCCTTTATTTCCTGATACGTGTCATTT10.5128205128205128No Hit
GATGAGTCATCGTCGAACATGCAACATTTACTGGATGATACGTTGCATTT10.5128205128205128No Hit
TACCCTTACTATGTTCCTGATTACGCGGCTCTGGGATTGCTTGTTGCTTC10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGGGCGATAATATTTCTTTGGT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCCAACGATTTGGTTCGGGACTCTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCTCGATGATTTCGTTGGGGA10.5128205128205128No Hit
ACGCCCAAGCAGATCTACGTTGAGCCGCAAGTGACTGTCTCTTATACACA10.5128205128205128No Hit
ACACCCAAGCAGATCTACCACAGCCCGGTTCAACCGCAAGTGACTGTCTC10.5128205128205128No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA10.5128205128205128No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATGTCCTGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCACGTCGAACAGTGGCACTTGCGCAGCCGCAGGCGGGCCGATC10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACAATTCTTGACCCGTATGTCTCCGATT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCGCTAAAATTTCCTTGGT10.5128205128205128No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGGGGCATTT10.5128205128205128No Hit
GATGCGTCAGCGACTAACATGCACCATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGGGGCATTT10.5128205128205128No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTGGGTTACTTTG10.5128205128205128No Hit
TACCCTTACTATGTTCCTGATTACGCATAACTCGATGCCATCTCTCATAA10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCATCATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGAAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGACACGTGGCATTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG10.5128205128205128No Hit
TACCCTTACGCTGTTCCTGCTTACGGTTCGTGTCTCGAATATTGCGTGGT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGTCTGGAGTATTGGGTTGG10.5128205128205128No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTGGCCAGCTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGTCTCGAATATTATGTTGG10.5128205128205128No Hit
GATGCGTCCGCGTCTAACAGTGGCACTTCCGCAGCCGCATTCGGTCCGAT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGGATGGAACAGGGGTTGACTTG10.5128205128205128No Hit
ACGCCCAAGCAGATCACGTTGAGCCGCAAGTGACTGTCTCTTATACACAT10.5128205128205128No Hit
GATGAGTCAGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
ACGCCCAAGCAGTGAGCCGCAAGTGACTGTCTCTTATACACATCTCCGAG10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTATGCTCTAAGAGTTCGTG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCGGGTCTGGTTCGGTTGGTTTCGTT10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTTGTATTGACTCG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTCGTTGGGGACTCTG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTAGTTACCACAGCTTAACCCTCCGGCCTC10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC10.5128205128205128No Hit
GATGAGTCAACGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACAGTGGCACTTGCGCAGCCGCAGGCGGGCCGAT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCGAGGTCCCAGACATCGCGGCGGA10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATATAGTCGAGCCAGACGAGTTTTCGTTAGT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACACTGGAATGAAGAAGCAGGCTATGTGTGGATG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTTGCTCTAAGATTTTGTT10.5128205128205128No Hit
ACGCCCAAGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTGAGC10.5128205128205128Illumina Single End PCR Primer 1 (96% over 33bp)
CTGTACAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.5128205128205128No Hit
ACGCCCAAGCAGTTGAGCCGCAAGTGACTGTCTCTTATACACATCTCCGA10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCTCCACGATTTCGTTTGGGACTCTT10.5128205128205128No Hit
GATGAGTCAGCGTCGATAACGTATGAACCGAATCGCTGTCAGCGCCCATG10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTCTGACAATGCTCAACCCTTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGCTAACGACTATCCATCTGTTTCGGGACTCAT10.5128205128205128No Hit
ACGCCCAAGCAGAGCCGCAAGTGACTGTCTCTTATACACATCTCCGAGCC10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGGGCTCTAAGATTTCTTTGGT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCCTTG10.5128205128205128No Hit
GCTGTGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT10.5128205128205128No Hit
ACACCCAAGCAGCTTTACAACGACCCGGCCAACCTGTTCTGCGCCAGTTT10.5128205128205128No Hit
CTGTAAAGATAAATGGGTCATCTAACTCTTATGTCTCTGTTCTTGACTCT10.5128205128205128No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT10.5128205128205128No Hit
GATGCGTCCGCGTCTAACATGAACCCTCTATTGCCTGATACGTGTCATTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGTTCTCGAATCGTTCGTTG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGTTTACGCTACAGGATTTTGTTTGGGACTCTT10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTCG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGCAGTTTTCGTTGGG10.5128205128205128No Hit
TACCCTTACGATGTTCCTGATTACGTCAGTTGACAAAGCTGAACCCTTCT10.5128205128205128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)