FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_AllHamPathoPool2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_AllHamPathoPool2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT4432.83582089552239No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG2619.402985074626866No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT32.2388059701492535No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG21.4925373134328357No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG21.4925373134328357No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT21.4925373134328357No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATTCCGATCGTCACCTTCGT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG10.7462686567164178No Hit
GAGGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATCT10.7462686567164178No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
AATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCCTAGGAGTTTGCCAGGCCGCCTAA10.7462686567164178No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTCGGATTGACTCG10.7462686567164178No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTTCTTGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGAGCTTTGGTTTCGGACTCTA10.7462686567164178No Hit
CTGTAAAGATAAATGGGCCCTCTAAAGAGTATGGAGCTGGGATTGACTCG10.7462686567164178No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.7462686567164178No Hit
GATGCGTCCGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGAGGCTCGAAGGTTTCCTTGG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTCGCGCGATGTTTTTGGTGG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATAACGACTAGCTAGCCGATTGGGGGCAAAT10.7462686567164178No Hit
GATGAGTCAGCGGTCGAACATGCAACATTTATTGCATGATACGTGGCATT10.7462686567164178No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTCAATGTGCTCGTTGGGCGCCTTT10.7462686567164178No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.7462686567164178No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGCTTCTCGAATCCTTCGGTG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGCTTACGCTGGGCTCGAAGATTTCGTTGGGGA10.7462686567164178No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTATAATATTGCTCTGCT10.7462686567164178No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGGATGGAGCTGGTATTGACTCG10.7462686567164178No Hit
GAAGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
CTGTAAAGATAAATGGGTCATATAAAGAGTATGGAGCTGGGATTGACTCG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCGGAGTTCGCGGGGGG10.7462686567164178No Hit
GAGGAAGGAATCGTTCCTGGCGGCGGCGTAGCCCTGCTGCGCGCTCGTGC10.7462686567164178No Hit
GAGGAGGGCATCGTTCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCGCTTTGGTTTGGGTCTCTT10.7462686567164178No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGACTACGCTCTACGATTTCGTTTGGGAATCTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTATTACGCCCTGGGCTCGAAGATTTCGGTGGGT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTAGGTCCGAAGATGCCGGTCTGT10.7462686567164178No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTGGAAGGTTTCGTTGGGG10.7462686567164178No Hit
GATGAGTCAGCGTCGAACACTGGAATGAAGAAGCAGGCTATGTGTGGATG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTTGG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCATTGGTGACAAAGTTTAACCCTTC10.7462686567164178No Hit
GATGAGTCAGCGTTGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
GATGAGTCAACGTCGAACATGCAACATTTATTGCATGATACGTGGCATTA10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCCTCGAATCTTTCGTTG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCGTATGGGGATCGTAGATTTAGTTG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCGTCAAAGCGCTCTAAGTCTTTGTT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTCTGTGCCAGCCCGCCTCG10.7462686567164178No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.7462686567164178No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTAACTCATTGCTTTCGCTCCTAA10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCATAATCCAGAGCCATCCCTCCTAA10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGTTTCTCGAATGATTCGGTG10.7462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCCGCGGGCCTGAAGACTTCGTTGGC10.7462686567164178No Hit
GATGAGTCAGCGTCTAACATGCAACATTGATTGCATGATACGTGGCATTG10.7462686567164178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)