FastQCFastQC Report
Tue 5 Sep 2023
000000000-L42GL_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L42GL_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000836
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTCCC295395521.09841869442653Illumina Single End PCR Primer 1 (100% over 8bp)
CTAGTATG5431983.879754037544615No Hit
GTCTAGTG4540173.2427849308427006No Hit
GATAGCGT4503253.2164150769282633No Hit
ACGCGTGA4212663.0088631850269514No Hit
TCTACACT4130222.949980986849642Illumina Single End PCR Primer 1 (100% over 8bp)
CGATCTAC3917832.7982829025352487No Hit
AAGCAGCA3841932.7440718539950044No Hit
CGTTACTA3711312.6507774250051925No Hit
CGTCGCTA3633682.595330735964624No Hit
CTACTATA3405882.432626166037514No Hit
GCTCTAGT3402252.4300334637160237No Hit
CTAGAGCT3401312.4293620752360785No Hit
GACACTGA3376302.4114988562111574No Hit
TGCGTCAC3374912.4105060583525155No Hit
TCGACGAG3357872.398335356545852No Hit
AGAGTCAC3337092.3834933856806835No Hit
TACGAGAC3212162.2942629997237307TruSeq Adapter, Index 14 (100% over 8bp)
TGCGTACG3127962.2341237337541844No Hit
ACGTCTCG2979012.127737229405444No Hit
ATATACAC2912672.080354344554854No Hit
ACGACGTG2724271.9457909513403342No Hit
TAGTGTAG2270581.6217460157379175No Hit
TAGCGAGT2222961.5877337610411264No Hit
TCATCGAG2180821.5576355583338024No Hit
CGTGAGTG2100801.5004818283708201No Hit
CTGCGTGT2089741.4925823000855092No Hit
ATCGTACG1969431.4066517170831798RNA PCR Primer, Index 22 (100% over 8bp)
GATCGTGT1948131.3914383398248504No Hit
ACTATCTG1927271.3765392295145804No Hit
GTCAGATA1724331.2315907421528258No Hit
GGATATCT1505141.0750358050047868No Hit
GACACCGT1497591.0696432698733132No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)