FastQCFastQC Report
Tue 5 Sep 2023
000000000-L42GL_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L42GL_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000836
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCTGA3170802.264721906606148No Hit
CGTCATAC3156322.2543796670427394No Hit
TCAGTCTA2991132.1363938553383526No Hit
CTCGACTT2933902.0955177247987193No Hit
AGCATACC2848602.0345927914590245No Hit
CGAAGTAT2827832.0197579630244937No Hit
GATCTACG2791491.9938023700870433No Hit
GACATAGT2785101.9892383569095444No Hit
CAGTAGGT2738251.9557760693718576No Hit
TAGCAGCT2726101.9470980161470357No Hit
GTAACGAG2722911.9448195807736053No Hit
CGTAGCGA2684751.9175640654600912No Hit
ATAGCGCT2606831.8619102459310286No Hit
TAGTCTCC2604771.8604389052196597No Hit
CATCGTGA2600071.8570819628199347No Hit
CGAGAGTT2581781.8440184571835567TruSeq Adapter, Index 13 (100% over 8bp)
CGAGCGAC2561471.8295121805583607No Hit
GTCTGCTA2560871.829083634720098No Hit
TCTAGACT2496801.7833220816242688No Hit
CGAGCTAG2495941.7827078325894252No Hit
GTCTATGA2426731.733275070145811No Hit
TGAGTACG2424841.7319251507552833No Hit
CTCTAGAG2382811.7019055147849742No Hit
TCTCTATG2362751.6875777989257212ABI Solid3 Adapter B (100% over 8bp)
ATGAGCTC2312471.6516656576792985No Hit
ACGCTACT2289201.6350452215853395No Hit
ACTCACTG2281051.6292241406156032No Hit
CTGCGTAG2189621.5639208972949898No Hit
TATAGCGA2172631.551785907641515No Hit
TGCTCGTA2150891.5362582634351263No Hit
ACTACGAC2070941.479154530486608No Hit
GCGTATAC1985431.4180796061035212No Hit
GAGCTCGA1955961.3970308630141801No Hit
TCCTCATG1862911.3305705459302573No Hit
ACGTGCGC1782661.2732525400626078No Hit
ATAGTACC1528901.0920062201999936No Hit
CTACGACC1284510.9174523578449173No Hit
GTTACAGC1200490.8574416556268498No Hit
GAGACTTA1168450.8345573078636161No Hit
TAACGTCC1135610.8111015656493655No Hit
ACGTACGT1066460.7617116577895777No Hit
AGTCGCAG1064250.760133180618643No Hit
ACTGTGTA1052520.7517551094806053No Hit
CGCGATAT917030.6549823167702271No Hit
GCGATACG896550.6403546188241902No Hit
TACTAGGT887960.6342192709063945No Hit
CTATCGTG740980.5292398253932837No Hit
TGCGTCAA630240.450144548511246No Hit
CTCTCTCT170270.12161416646834518No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)