FastQCFastQC Report
Thu 4 May 2023
000000000-L387F_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L387F_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14537829
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCA1361715993.6670736737927No Hit
TGACAA1319850.9078728330069091No Hit
TTACCA439550.302349133422879No Hit
TGACCC329270.2264918647756828No Hit
TTCCCC316260.21754279817158395No Hit
TTACCC194380.13370634638775844No Hit
TTCCCA159000.10936983782103918No Hit
GGAAAA155330.10684538936315732No Hit
GACCAA146290.10062712940150831No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)