FastQCFastQC Report
Tue 14 Feb 2023
000000000-KWL6M_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWL6M_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295794
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC343712.652504950632585No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC330402.5497880064269474No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA323942.499934403153588No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC134781.0401344658178693No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC100950.7790590170968533No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCC53200.41055908578061023No Hit
GATCGATAACGCATTTCTTGTGGACAGGACGACACACCGCCAGTAGGTCC15280.1179199780211978No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCCA14030.10827338296056319No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGTTCC13030.10055610691205547No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)