FastQCFastQC Report
Tue 14 Feb 2023
000000000-KWL6M_l01_n01_GibsonORF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWL6M_l01_n01_GibsonORF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990356
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC35931718.052901089051407No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA32765916.462331361826728No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC25858312.991796442445471No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC1386106.964080797606057No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC1207816.06831139755903No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCC349481.7558667896597393No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCCA168200.8450749514157265No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGAAACACCGCCAGTAGGTCCAC130750.6569176569417732No Hit
ATCGATTCCTTGGTTCTTGTGGACAGGACGACACACCGCCAGTAGGTCCA124860.6273249609617576No Hit
CGATCGATCCAGGTATTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC113950.5725106463366353No Hit
ATCGATTCCTTGGTTCTTGTGGACAGGACGAAACACCGCCAGTAGGTCCA95010.4773517903329857No Hit
CGATCGATACAGGTACTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC93600.4702676305143401No Hit
GATCGATAACGCATTTCTTGTGGACAGGACGACACACCGCCAGTAGGTCC69920.3512939393756695No Hit
CGATCGATCCAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC45980.2310139492633479No Hit
CGATCGATATAGGTACTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC44580.22398003171291972No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAAGTAGGTCCA35540.17856102124444068No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCCGTAGGTCC34440.17303437174053285No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGCAACACCGCCAGTAGGTCCAC31960.16057428922263153No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTCGGTCC31840.15997138200402342No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCCGTCGGTCC31320.15735878405672152No Hit
CGATCGATACAGGTAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC30170.15158092321172695No Hit
GATCGATAACGCATTTCTTGTGGACAGGACGAAACACCGCCAGTAGGTCC28080.14108028915430204No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGCAACACCGCCAGTAGGTCCAC26020.13073038190152916No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTATGTCC23820.11967708289371348No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAAGTAGGTC21780.10942766017737529No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGCAACACCGCCAGTATGTCCAC21680.10892523749520186No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT21360.10731748491224687No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAA21230.10666433542542138No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGAAACACCGCCAGTATGTCCAC20170.10133865499438292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)