FastQCFastQC Report
Tue 14 Feb 2023
000000000-KWL6M_l01_n01_CMVORF.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWL6M_l01_n01_CMVORF.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886219
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA33631017.82984902601448No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC31888116.905831189273357No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC24026612.737969451055259No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC1121165.943954546105198No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC1063425.637839508561838No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCC333661.7689356326068182No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGACACACCGCCAGTAGGTCCA165900.8795373177769918No Hit
ATCGATTCCTTGGTTCTTGTGGACAGGACGACACACCGCCAGTAGGTCCA126980.6731986052520943No Hit
CGATCGATCCAGGTATTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC104480.553912350580712No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGAAACACCGCCAGTAGGTCCAC103920.5509434482422243No Hit
ATCGATTCCTTGGTTCTTGTGGACAGGACGAAACACCGCCAGTAGGTCCA92630.491088256453784No Hit
CGATCGATACAGGTACTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC86490.45853636295679345No Hit
GATCGATAACGCATTTCTTGTGGACAGGACGACACACCGCCAGTAGGTCC65330.3463542674525068No Hit
CGATCGATCCAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC43080.2283934156108066No Hit
CGATCGATATAGGTACTCTTGTGGAAAGGACGACACACCGCCAGTAGGTC40830.21646479014366835No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAAGTAGGTCCA37350.19801518275449456No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCCGTAGGTCC34310.18189828434556113No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTCGGTCC30160.15989659737283954No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCCGTCGGTCC28460.15088385813100175No Hit
GATCGATAACGCATTTCTTGTGGACAGGACGAAACACCGCCAGTAGGTCC26270.13927332934298722No Hit
CGATCGATACAGGTAGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC26020.13794792651330517No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGCAACACCGCCAGTAGGTCCAC25050.13280536353413894No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTATGTCC23070.12230817312305728No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAA22590.11976339969006779No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGTTCCA21390.11340146610759408No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAAGTAGGTC20770.11011446708998267No Hit
TCGATCGTTACCATCTTGTGTAAAGGACGCAACACCGCCAGTAGGTCCAC20660.10953128984492255No Hit
ATCGATTCCTTGGTTCTTGTGGATAGGACGACACACCGCCAGTAGGTCCA19300.10232109845145236No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)