FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4H9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4H9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA104993.16163410301954No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA262.3090586145648313No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.3552397868561279No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.3552397868561279No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.3552397868561279No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA30.2664298401420959No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTATCCATACTAGTTAGAGAAC30.2664298401420959No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA20.17761989342806395No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA20.17761989342806395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)