FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4H3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4H3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA327093.00341296928327No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA842.3890784982935154No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA90.25597269624573377No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA90.25597269624573377No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.25597269624573377No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA50.1422070534698521No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11376564277588168No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11376564277588168No Hit
TGAGTAACCGCAACACAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA40.11376564277588168No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA40.11376564277588168No Hit
TAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTGGTTAGAGAA40.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)