FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4H1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4H1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA180392.31950844854072No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA442.252944188428059No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA140.7168458781362007No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA70.35842293906810035No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA70.35842293906810035No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.2048131080389145No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA40.2048131080389145No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.15360983102918588No Hit
GTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA30.15360983102918588No Hit
TGAGTTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA30.15360983102918588No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACATAGTGAAATCACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACCTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TAAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACATAGTTAAAACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCGACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACATAGTAAAAACACAGTTATCCATACTAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACCAGTTAGAGAA20.10240655401945725No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA20.10240655401945725No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)