FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20784.48979591836735No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA72.857142857142857No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA41.6326530612244898No Hit
TGAGTAACCGCAACATAGCGAAAACACAGTTATCCATACTAGTTAGAGAA20.8163265306122449No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTCAGAGAA20.8163265306122449No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGAGCCATACGAGTTAGAGAA20.8163265306122449No Hit
AGAGTAACCGCAACAGAGGGAAAACACAGTGAGCAAGACGAGTGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATAGGAGGGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACGAGTGAGAGAA10.40816326530612246No Hit
AGACTACCCAAGCGGCATAGCCTGAAATAGAACATAACATAGAGTAACGG10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGGGAAAACACAGATAGCCATACGAGGGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTTAAAACACAGTTATCCATACTAGTTAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACGAGTTAGAGAA10.40816326530612246No Hit
AGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA10.40816326530612246No Hit
GGAGGAACCGCAACAGAGTGAAAACACAGGGAGCAATACGAGTGAGAGAA10.40816326530612246No Hit
CACCACCGACTACGAACAATGTGAAAGTTAATCAATAGAAGTAATAGCTT10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCAATACTAGGTAGAGAA10.40816326530612246No Hit
TGAGGAACCGCAACAGAGTGAAAACACAGGGAGCCATACGAGTGAGAGAA10.40816326530612246No Hit
GGAGGAACCGCAACAGAGTGAAAACACAGTGAGCAATACGAGTGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACCAGTTAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGGGAAAACACAGTTAGCCATACTAGTGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA10.40816326530612246No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACGAGGGAGAGAA10.40816326530612246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)