FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA85590.18987341772153No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA222.320675105485232No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA90.949367088607595No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA40.42194092827004215No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA40.42194092827004215No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA30.31645569620253167No Hit
TGAGTAACCGCGACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.31645569620253167No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA20.21097046413502107No Hit
TGGTAACCGCAAATAGTGAAAACACAGTTATCCATACTAGTTAGAGAACC20.21097046413502107No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA20.21097046413502107No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA20.21097046413502107No Hit
TGAGTAACCGCAAATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.21097046413502107No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.21097046413502107No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.21097046413502107No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGATAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCTCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
GAAAGGTCATCCGGCTTACGCACGGCGCCAGATCGAATATTAGACATAAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTCGAGAA10.10548523206751054No Hit
TACTAACAAGCCCCTTGGAAGGCATAGGTATAAAACAAATAGATCAACAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCTATACTATTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGCA10.10548523206751054No Hit
AGCGGTATCGCACCTCACTGATTACAGATATTGCAATGGTAGTTTGTGAT10.10548523206751054No Hit
TGAGTAATCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCCACATAGTGAAAACAACGTTATCCCTACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAC10.10548523206751054No Hit
TAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGATAACCTT10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTGAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCACATAGTGAAAACACAGGTATCCATACTAGTTAGGGAAC10.10548523206751054No Hit
TGAGTAACGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.10548523206751054No Hit
TGCGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTACCCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCCACATAGTGAAAACACAGGTATCCATACTTGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCTCCACATAGTGAAAACACAGTTATCCATACTAGTTAGATAA10.10548523206751054No Hit
TGAGTAACCACAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAACCTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGATAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAACC10.10548523206751054No Hit
TGGTAACCGCCACCTAGTGAAAAACCAGTTATCCATACTAGTTAGGGAAC10.10548523206751054No Hit
AAACTAATCAGAATCAGGATAAGCCGCAACTACGGGATCAAGATACTCAC10.10548523206751054No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTCGAGAA10.10548523206751054No Hit
TGATTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGCAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTAACCGCAAATAGTTAAAACACAGTTATCCATACTAGTTAGAGAAC10.10548523206751054No Hit
TGAGTTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.10548523206751054No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)