FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4G9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4G9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA146692.20125786163523No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA382.3899371069182394No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA70.44025157232704404No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.37735849056603776No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.3144654088050315No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA40.25157232704402516No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA30.18867924528301888No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA30.18867924528301888No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTTGAGAA30.18867924528301888No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.18867924528301888No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA30.18867924528301888No Hit
TGAGTAAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA20.12578616352201258No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA20.12578616352201258No Hit
TGAGTAACCGCAACATAGTGAATACACAGTTATCCATACTAGTTAGAGAA20.12578616352201258No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATCCTAGTTAGAGAA20.12578616352201258No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA20.12578616352201258No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.12578616352201258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)