FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4G1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4G1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA242892.99119111451552No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA451.7234775947912677No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA200.7659900421294523No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA70.2680965147453083No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA60.2297970126388357No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA50.19149751053236308No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.19149751053236308No Hit
TGAGTAAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA40.15319800842589046No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA40.15319800842589046No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA40.15319800842589046No Hit
TAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.15319800842589046No Hit
TGAGTAACCACAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.11489850631941785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA30.11489850631941785No Hit
TGAGTAACCGCAACCTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.11489850631941785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCTTACTAGTTAGAGAA30.11489850631941785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATCCTAGTTAGAGAA30.11489850631941785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA30.11489850631941785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)