FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA19690.32258064516128No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA52.3041474654377883No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA20.9216589861751152No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGACAGACGAGGGAGAGAA10.4608294930875576No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTCTCCATACTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGGGAGCCATACGAGTGAGAGAA10.4608294930875576No Hit
TGAATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.4608294930875576No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGGTATCAATACTAGTTAGAGAA10.4608294930875576No Hit
GGAGTAACCGCAACAGAGTGAAAACACAGGTAGCCATACGAGGGAGAGAA10.4608294930875576No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTAAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGATCCATACTAGTGAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATCCTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA10.4608294930875576No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTACCCATACTAGTTAGAGAA10.4608294930875576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)