FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4F7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4F7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA396391.86369958275382No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA851.9703291608715807No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA360.8344923504867872No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA150.34770514603616137No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA140.3245248029670839No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA90.2086230876216968No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA80.18544274455261936No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.16226240148354196No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA70.16226240148354196No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA70.16226240148354196No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA50.11590171534538712No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA50.11590171534538712No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA50.11590171534538712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)