FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4193
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA386592.17743858812307No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1052.5041736227045077No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA130.3100405437634152No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC110.26234199856904367No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA100.23849272597185783No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.1669449081803005No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.1669449081803005No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.14309563558311472No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA60.14309563558311472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)