FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA182692.54941713127216No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA522.6355803345159656No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC90.456158134820071No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA60.30410542321338063No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.25342118601115055No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA40.20273694880892043No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA40.20273694880892043No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.20273694880892043No Hit
TGAGTAACCGCAAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA30.15205271160669032No Hit
GTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA30.15205271160669032No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.15205271160669032No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA30.15205271160669032No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.15205271160669032No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTTTCCATACTAGTTAGAGAA20.10136847440446022No Hit
TGAGTAACCGCAACATAGAGAAAACACAGTTATCCATACTAGTTAGAGAA20.10136847440446022No Hit
TGACTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.10136847440446022No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA20.10136847440446022No Hit
TGAGTAACCGAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.10136847440446022No Hit
TGAGTAACGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.10136847440446022No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA20.10136847440446022No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)