FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4E12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4E12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA47688.31168831168831No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA40.7421150278293136No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTGAGAGAA30.5565862708719851No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.5565862708719851No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA20.3710575139146568No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA20.3710575139146568No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA20.3710575139146568No Hit
TGAGTAACGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.3710575139146568No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTTTCCATAACAGGTTGAGAA10.1855287569573284No Hit
TGAGTACCGCAACATAGGTAAAACACAGTTTTCCATTCTTAGTAGAGGAC10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTAGCCATACTAGGTAGAGAA10.1855287569573284No Hit
GGAGGAACCGCAACAGAGGGAAAACACAGTGAGCCAGAGGAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGAGATCCATAATAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA10.1855287569573284No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTAGCAATACGAGTGAGAGAA10.1855287569573284No Hit
TGAGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.1855287569573284No Hit
TGCGTCACCGCAACATAGCGTTAATACAGTTATCCATACTAACTTGTGAA10.1855287569573284No Hit
TGAGTAACCCCACATTGTGAAAAAACCATTTTCCCTTATAGTTAGAGGAA10.1855287569573284No Hit
GAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.1855287569573284No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGATCCAGACGAGGGAGAGAA10.1855287569573284No Hit
AGAGGAACCGCAACAGAGTGAAAACACAGTGAGCCATACTAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGGAGCCATACGAGTTAGAGAA10.1855287569573284No Hit
GGAGTAACCGCAACATAGTGAAAACACAGGGAGCCAGACGAGTAAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTAGCCATACTAGTTAGAGAA10.1855287569573284No Hit
TGGTAACCGCCACCTTGTGAAAACACAGGTTTTCATTCTAGTTAGAGAAA10.1855287569573284No Hit
GGAGTAACCGCAACAGAGTGAAAACACAGTTAGCAATACGAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCAGACGAGGGAGAGAA10.1855287569573284No Hit
TCAGTACCCCCAACATCGTGAACATACATTTATCCAATCTCGTTACAGAA10.1855287569573284No Hit
TGAGTACCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.1855287569573284No Hit
TGCGTAACCGCACCCTAGTGACCACACAGTTCTCAATACTAGTTCGAAAA10.1855287569573284No Hit
TGAGGAACCGCAACATAGTGAAAACACAGTGAGCCATACGAGTGAGAGAA10.1855287569573284No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGGGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGATACATACTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGGGAAAACACAGGGATCCATACGAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGGTAGAGAA10.1855287569573284No Hit
TGAGTAACCCCACCTAATTAAAACACAATTAACCCATCTAGTTAGAGAAC10.1855287569573284No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGAGCCATACTAGTGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACAGAGGGAAAACACAGTTATCCATACTAGGGAGAGAA10.1855287569573284No Hit
TGAGTAACGGAAAAAAGGGGAAAAAAAGGGAAGCAAAACAGGAAGAGGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA10.1855287569573284No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGGGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATTCATACTAGTTAGAGAAC10.1855287569573284No Hit
TGCGTAACCGCAACATAGTGAAAACACAGCTATCCATACTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACAGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTAGCCATACTAGAGAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA10.1855287569573284No Hit
TGATTAACCGCACCTTAGTGAAAACACAGTTACCCATATTAGTTAGAGAA10.1855287569573284No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGAGCCATACTAGTTAGAGAA10.1855287569573284No Hit
GGAGTAACCGCAACAGAGTGAAAACACAGGGAGCCATACGAGAGAGAGAA10.1855287569573284No Hit
TGTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAG10.1855287569573284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)