FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA82693.97042093287827No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.9101251422070534No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA40.4550625711035267No Hit
GTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA30.3412969283276451No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.22753128555176336No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA20.22753128555176336No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.22753128555176336No Hit
TGAGTAACCGCAACATAGTGAGAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGATAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA10.11376564277588168No Hit
TTAAGGTAATCTACCTTTTTACCACAATGATAGTAAAAAACTAGAAAAAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTTGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTTTACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCTCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCTCCACCTAGTGACAACACCGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCCCAACATAGTGACAACACAGTTATCCCTACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACATAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
AGTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAG10.11376564277588168No Hit
TGAGTAAACGCCACCTAGTTAAAAAACAGTTATCACTAATATTTTGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGATAA10.11376564277588168No Hit
TGAGGCACCGCCACATAGTGCAAACACAGGTATCCATACTATGTAGAGAA10.11376564277588168No Hit
TAAGAGTTTGCAATATGGCGAGAACTCAGAGACTGAAACATGATTGAGCA10.11376564277588168No Hit
TGAGTAACCGCAACTAAGTGAAACAACAGTAATCAATCTTGGTTAGGGAC10.11376564277588168No Hit
TGAGTAACCGCAAAATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCTCAAAATAGTGAAAACACCGTTATCCCTACTAGTTTGAGAA10.11376564277588168No Hit
TGAGTAAAAGAAATATAGATGATATATAGTGATACAATCGAGTAATACTC10.11376564277588168No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTGGAGAA10.11376564277588168No Hit
TGAAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAC10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTTGTTAGAGAA10.11376564277588168No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCGATACTAGTTAGAGAA10.11376564277588168No Hit
TTAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTCGTTAGAAAA10.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)