FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4D12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4D12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA106285.8528698464026No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA151.2126111560226354No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.6467259498787389No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA60.4850444624090542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA60.4850444624090542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA40.32336297493936944No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA40.32336297493936944No Hit
TGAGTAACCGCAACTAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.32336297493936944No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA40.32336297493936944No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA40.32336297493936944No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA30.2425222312045271No Hit
TGAGTAACCGCAAATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.2425222312045271No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA30.2425222312045271No Hit
GAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA20.16168148746968472No Hit
TGAGTAACCGCAACTTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGGTAGAGAA20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA20.16168148746968472No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.16168148746968472No Hit
TGAGTAACCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA20.16168148746968472No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGGTATCCATACGAGTGAGAGAA20.16168148746968472No Hit
AGAGTAACCGCAACATAGTGAAAACACAGGTAGCCATACGAGTGAGAGAA20.16168148746968472No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTACCCATACTAGTTAGAGAA20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTAATCCATACTAGTTAGAGAA20.16168148746968472No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTGAGAGAA20.16168148746968472No Hit
TGAGTAACGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.16168148746968472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)