FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4C3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4C3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5638
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA522992.74565448740688No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1272.252571833983682No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA240.4256828662646328No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA170.3015253636041149No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA130.23057821922667612No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA90.15963107484923733No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.15963107484923733No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA80.1418942887548776No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA70.12415750266051792No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA60.1064207165661582No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCGTACTAGTTAGAGAA60.1064207165661582No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.1064207165661582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)