FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4704
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA425690.47619047619048No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1182.508503401360544No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA250.5314625850340136No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA160.3401360544217687No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA150.31887755102040816No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA140.2976190476190476No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA100.21258503401360546No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.1913265306122449No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.1913265306122449No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA80.17006802721088435No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.1488095238095238No Hit
TGAGTAACCACAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.1488095238095238No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA60.12755102040816327No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTCGAGAA60.12755102040816327No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA60.12755102040816327No Hit
GTGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA50.10629251700680273No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAACCATACTAGTTAGAGAA50.10629251700680273No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA50.10629251700680273No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.10629251700680273No Hit
TGAGTAACCTCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.10629251700680273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)