FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP4A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP4A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA274991.69446297531688No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA772.5683789192795197No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA150.5003335557038026No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA70.23348899266177453No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA60.20013342228152103No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA60.20013342228152103No Hit
TAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.1667778519012675No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.1667778519012675No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.13342228152101399No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA40.13342228152101399No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.13342228152101399No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA40.13342228152101399No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCTTACTAGTTAGAGAA40.13342228152101399No Hit
TGATTAATCTCAACATAGTTAAAACACAGTTATCCATACTAGTTAGAGAA40.13342228152101399No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.10006671114076052No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTATAGAA30.10006671114076052No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTTGAGAA30.10006671114076052No Hit
TGAGTAACCGCAACATAGTGAAAACACGGTTATCCATACTAGTTAGAGAA30.10006671114076052No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTAAGAGAA30.10006671114076052No Hit
TGAGTAACCGCAAATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.10006671114076052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)