FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3599
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA317888.3023061961656No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA651.8060572381217006No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA150.41678243956654626No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA130.3612114476243401No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA120.333425951653237No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC100.27785495971103086No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA80.22228396776882464No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA80.22228396776882464No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.1667129758266185No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTTAGAGAA50.13892747985551543No Hit
TAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.13892747985551543No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.13892747985551543No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA40.11114198388441232No Hit
TAAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.11114198388441232No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCGATACTAGTTAGAGAA40.11114198388441232No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACGAGTGAGAGAA40.11114198388441232No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA40.11114198388441232No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA40.11114198388441232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)