FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2791
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA261493.65818702973844No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA441.5764958796130417No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA90.32246506628448585No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.2508061626657112No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.2508061626657112No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA60.21497671085632392No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA50.17914725904693657No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA50.17914725904693657No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.17914725904693657No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA40.14331780723754925No Hit
TGAGTAACCGCGACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.14331780723754925No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTACCCATACTAGTTAGAGAA30.10748835542816196No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA30.10748835542816196No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.10748835542816196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)