FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA327487.63383297644539No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA842.2483940042826553No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA350.936830835117773No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA230.6156316916488223No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA180.4817987152034261No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA150.4014989293361884No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA130.34796573875802994No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA130.34796573875802994No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA120.32119914346895073No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA110.2944325481798715No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA110.2944325481798715No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA100.26766595289079226No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.24089935760171305No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA90.24089935760171305No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA70.1873661670235546No Hit
TGAGTAACCGCAACATAGTGAAAACTCAGTTATCCATACTAGTTAGAGAA60.16059957173447537No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.13383297644539613No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTCTCCATACTAGTTAGAGAA50.13383297644539613No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.13383297644539613No Hit
TGAGTAACCGCAACCTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.10706638115631692No Hit
TGAGTACCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.10706638115631692No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTCGAGAA40.10706638115631692No Hit
TGAGTAACCGCAACATAGTGAAAACCCAGTTATCCATACTAGTTAGAGAA40.10706638115631692No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA40.10706638115631692No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.10706638115631692No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATCCTAGTTAGAGAA40.10706638115631692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)