FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA322487.53733369535705No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA701.9006244909041543No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA160.4344284550638067No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA140.38012489818083084No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA140.38012489818083084No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA110.2986695628563671No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA100.27151778441487917No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA80.21721422753190334No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA80.21721422753190334No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA80.21721422753190334No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTTAGAGAA70.19006244909041542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAG70.19006244909041542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA70.19006244909041542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA70.19006244909041542No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACGAGTTAGAGAA40.10860711376595167No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.10860711376595167No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTGAGAGAA40.10860711376595167No Hit
TGAGTATCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.10860711376595167No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA40.10860711376595167No Hit
TGAGTAACCGCAACATAGTAAAAACACAGTTATCCATACTAGTTAGAGAA40.10860711376595167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)