FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1682
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA148788.40665873959573No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA140.8323424494649228No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA80.47562425683709864No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA70.4161712247324614No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.356718192627824No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA40.23781212841854932No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA40.23781212841854932No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.178359096313912No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA30.178359096313912No Hit
TGAGTAACCGCAACGTAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.178359096313912No Hit
TGCGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC30.178359096313912No Hit
TGAGTAACCGCAACACAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.178359096313912No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA30.178359096313912No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGATAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA20.11890606420927466No Hit
TGAGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.11890606420927466No Hit
TGAGGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.11890606420927466No Hit
TGAGTAACTGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.11890606420927466No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.11890606420927466No Hit
TGAGTAACCGCAACATGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.11890606420927466No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTCAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGGTAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACGAGTGAGAGAA20.11890606420927466No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTGATCCATACGAGTGAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACGAGTGAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA20.11890606420927466No Hit
TGAGTAACCGCAACATTAGTGAAAACACAGTTATCCATACTAGTTAGAGA20.11890606420927466No Hit
TGAGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGTGAA20.11890606420927466No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCATACTAGTGAGAGAA20.11890606420927466No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)