FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA232392.80862964442669No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA542.1574111066719937No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA110.43947263284059124No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA110.43947263284059124No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA100.3995205753096284No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.2796644027167399No Hit
TGAGTAACGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.1997602876548142No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.15980823012385137No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA30.11985617259288853No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA30.11985617259288853No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA30.11985617259288853No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCGATACTAGTTAGAGAA30.11985617259288853No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA30.11985617259288853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)