FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA159693.55216881594373No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA352.0515826494724503No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA70.4103165298944901No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA50.29308323563892147No Hit
TGAGTAAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA40.23446658851113714No Hit
CGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.23446658851113714No Hit
TGAGTAACTGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.1758499413833529No Hit
TGAGTAACCGCAACATAGTGAAAACGCAGTTATCCATACTAGTTAGAGAA30.1758499413833529No Hit
TGAGTAACCGCGACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.1758499413833529No Hit
TGAGTAACCGCAACATAGTGATAACACAGTTATCCATACTAGTTAGAGAA20.11723329425556857No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.11723329425556857No Hit
TGAGTAGCCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.11723329425556857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)