FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA53887.05501618122977No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA71.132686084142395No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA60.9708737864077669No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA30.48543689320388345No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA30.48543689320388345No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA30.48543689320388345No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGCTAGAGAA20.3236245954692557No Hit
TGAGTAACCGCACCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.3236245954692557No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA20.3236245954692557No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA20.3236245954692557No Hit
AGAGGAACGGCAAGAGAGTGAAAGGAGGGGGAGGAATAGGGAGGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCAAACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAGCACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACATAGTGAAAACACAGAGATCAATACGAGGGAGAGAA10.16181229773462785No Hit
GGAGTAACCGCAACATAGTGAAAACACAGGTAGCCAGACTAGTGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTAAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGAGGAACCGCAACAGAGTGAAAACACAGAGAGCCAGACGAGGAAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGATCCAGACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCCTACTAGTTCGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCCACATAGTGAAAACACAGGGATCCATACTACACAGACAA10.16181229773462785No Hit
GGAGGAAACGCAACATAGTGAAAACACAGGGAGCCAGACGAGGGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAATCACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCACAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTAGCCAGACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA10.16181229773462785No Hit
TTAATAACCACACCATCTTTAACACACAGTTATCCATACTATTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACGAGTGAGAGAA10.16181229773462785No Hit
TGAGGAACCGCAACATAGTGAAAACACAGGTAGCCATAAGAGGGAGAGAA10.16181229773462785No Hit
TGAGTACCGGCACCATGGTGAAAACACGGTTATCCAAACTAGTAAGGGAA10.16181229773462785No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACGAGTGAGAGAA10.16181229773462785No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGGAGGAA10.16181229773462785No Hit
TGAGTAACCTCCACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGGAAACACAGTTATCCATACTCGTTAGAGAA10.16181229773462785No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.16181229773462785No Hit
TGAGTAACCTCAACATAGTGACAACACAGTTATCCATACTAGTTACATAA10.16181229773462785No Hit
TGATTAACCGCCACATAGTGACAACACAGTTATCCATACTAGTTAGAGAC10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGTAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACAGAGTGAAAACACAGTGAGCCATACGAGTGAGAGAA10.16181229773462785No Hit
GGAGTAACCGCAACATAGTGAAAACACAGTGAGCCATACGAGTGAGAGAA10.16181229773462785No Hit
GGAGGAACCGCAACAGAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA10.16181229773462785No Hit
GGAGTAACCGCAACAGAGTGAAAACACAGTGATCCATACTAGTGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTAAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGATAACCGCCACATAGTTAAAACCCCGTTTTTCAATCTTATTAGAGAAC10.16181229773462785No Hit
TGAGTAACCGCGACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGGGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTAGACATACGAGTGAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACGAGGTAGAGAA10.16181229773462785No Hit
TACGTAACCGCACCATATTGAAAACACAGTTATCCATACTAGTTAGAAAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA10.16181229773462785No Hit
TGCGTAACCGCAGCATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTAGCCAGACTAGGGAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTGAGCCATACTAGTGAGAGAA10.16181229773462785No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA10.16181229773462785No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit
TGAGTAACCGCAACATAGTGATAACACTGTTATCCATACTAGTTAGAGAA10.16181229773462785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)