FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3G6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3G6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA383790.36740461610928No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA1172.7555346208195948No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA190.4474799811587376No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA160.3768252472915685No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA140.3297220913801225No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA120.2826189354686764No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA90.2119642016015073No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT90.2119642016015073No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.18841262364578426No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA70.16486104569006124No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC70.16486104569006124No Hit
TGAGTCACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA70.16486104569006124No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA60.1413094677343382No Hit
TGAGTAACCGCAACATAGTGCAAACACAGTTATCCATACTAGTTAGAGAA60.1413094677343382No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCCTACTAGTTAGAGAA60.1413094677343382No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTCGAGAA50.11775788977861518No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA50.11775788977861518No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGCGAA50.11775788977861518No Hit
TGAGTAACCGCAACATAGTGAAACCACAGTTATCCATACTAGTTAGAGAA50.11775788977861518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)