FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3G4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3G4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA351787.01138050470064No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA641.5833745670460169No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA290.7174666006927264No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA200.49480455220188024No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA100.24740227610094012No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA90.2226620484908461No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACTAGTTAGAGAA80.1979218208807521No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT70.17318159327065807No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA70.17318159327065807No Hit
TGAGGAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.14844136566056407No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTTAGAGAA50.12370113805047006No Hit
TGAGTAACCGCAACATAGTGACAACACAGTTATCCATACTAGTTAGAGAA50.12370113805047006No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC50.12370113805047006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)