FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3G3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3G3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA54790.26402640264027No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA81.3201320132013201No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTT71.155115511551155No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT40.6600660066006601No Hit
TGAGTACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.33003300330033003No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC20.33003300330033003No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA20.33003300330033003No Hit
TGAGTAAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGA10.16501650165016502No Hit
AAAGCCACGTCATCACCGCCTTCCCACAGTTATCAATCCTCGTTAGCCTA10.16501650165016502No Hit
TGAGTAACCGCAACATCGTGATAACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.16501650165016502No Hit
TGGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAAC10.16501650165016502No Hit
TGAGTAACCGCAACAAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCAACATCGTGAAGACACACATCTCCAAACTAGTTATCGCA10.16501650165016502No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGAAGTCACCTT10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAGACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC10.16501650165016502No Hit
AAAGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAAACACCGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAACACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGTAAACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
CCTGCCTATTCTTTCCCCCCTTACTAGTGTTTGTGCCCAATCGTCACCTT10.16501650165016502No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCTCT10.16501650165016502No Hit
TGAGTAACCGCACCATAGTGAAACCCGAGTTATACATACAAGTTAGAGAC10.16501650165016502No Hit
TCCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TCAGTAACCGCCACATCGTCTAACCACCTTTATCCATACTCGTTCGAGAA10.16501650165016502No Hit
TCATTAACCGCTACGTAGTAGAAACAATGCTATAAATACCAATAAGAGGA10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATATTAGTTAGAGAA10.16501650165016502No Hit
AAAGCCACGGCAACCCCACCTTCCTACAGTTTGTCACAGTCATTCAACAT10.16501650165016502No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACAGGCAGTATCCTT10.16501650165016502No Hit
TAAGTAACCTCAACATAGTGATACTACGGTTATCCATACTCGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGGGAA10.16501650165016502No Hit
TACATCCCCTCTCCCTCTTTCCCCCCTTTTACAAACTGATCATTCGCTAC10.16501650165016502No Hit
TGAGTACCGCAACATTGCTAAAACACAGTTTTCCATTCTTGTTAGGGAAA10.16501650165016502No Hit
TGAGTAACCGCAACATCGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGCA10.16501650165016502No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCCGCAGTCACCTT10.16501650165016502No Hit
AAAGTCACCGCAACATCACCAACCCACAGTTATCCATCCTAATTAACGAT10.16501650165016502No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA10.16501650165016502No Hit
TCCTAAAAAAACCTACGGCTTCGCATGTGTACAAGCACATGAAGGACCCC10.16501650165016502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)