FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n02_ELP3F5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n02_ELP3F5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA311787.21320649132625No Hit
TGCGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA802.238388360380526No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTGAGAGAA220.6155567991046447No Hit
TGGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA170.4756575265808618No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCAATACTAGTTAGAGAA150.4196978175713486No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTCGTTAGAGAA130.36373810856183547No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTTAGAGAA120.33575825405707893No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTTAGAGAA80.22383883603805263No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTAGCCATACTAGTGAGAGAA70.195858981533296No Hit
AGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA60.16787912702853947No Hit
TGATAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC60.16787912702853947No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCTATACTAGTTAGAGAA50.13989927252378287No Hit
TGAGTAACCGCAACAGAGTGAAAACACAGTTATCCATACGAGTGAGAGAA50.13989927252378287No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACGAGTGAGAGAA40.11191941801902631No Hit
TGAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGGTAGAGAA40.11191941801902631No Hit
GAGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11191941801902631No Hit
TGAGTAACCGCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11191941801902631No Hit
TGAGTAACCGCAACATAGTGAAAACACGGTTATCCATACTAGTTAGAGAA40.11191941801902631No Hit
TGAGTAACCGCCACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAA40.11191941801902631No Hit
TGAGTAACCGCAACATAGTGAAAACACAGGTATCCATACTAGTGAGAGAA40.11191941801902631No Hit
TGGTAACCGCAACATAGTGAAAACACAGTTATCCATACTAGTTAGAGAAC40.11191941801902631No Hit
AAGGCCACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTT40.11191941801902631No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)